Acquisition of Single Cell Data in an Optical Microscope
Kristin Sott, Emma Eriksson and Mattias Goksör
from: Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis (Edited by: Keith E. Herold and Avraham Rasooly). Caister Academic Press, U.K. (2009)
Data acquired on a single cell level has become increasingly important in the understanding of cellular behaviour. Traditionally, research in life science has focused on studies using ensemble averaging techniques. Since most cell models are based on averaged results from populations of cells, information regarding any heterogenic behaviour within the population is lost. Recent studies at the single cell level have revealed surprisingly heterogeneous behaviour, and highlight the importance of gaining detailed single cell data to update the models on cellular dynamics. We here present an experimental platform for analysing protein dynamics in single cells with high spatial and temporal control. The experimental platform utilizes the combination of the imaging resolution of a fluorescence microscope, with the spatial and temporal control of single cells and its environment provided by optical manipulation and microfluidics, respectively. The experimental platform is evaluated on the high osmolarity glycerol (HOG) signalling pathway in single Saccharomyces cerevisiae during environmental stress read more ...