Microchip Gel Electrophoresis of DNA with Integrated Whole-column Detection
Roger C. Lo and Victor M. Ugaz
from: Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis (Edited by: Keith E. Herold and Avraham Rasooly). Caister Academic Press, U.K. (2009)
Gel electrophoresis is an essential analytical step in a wide spectrum of DNA analysis assays. This importance has motivated efforts aimed at developing advanced microfluidic "lab-on-a-chip" systems that incorporate embedded electrophoresis capabilities. In this chapter, we describe recent work aimed at constructing a new automated whole-gel scanning detection system that allows microchip-based gel electrophoresis of DNA to be performed in a rapid, low-field, miniaturized format. This system is based on a versatile microfluidic platform that incorporates integrated on-chip electrodes, heaters, and temperature sensors coupled with instrumentation configured to enable the progress of a DNA separation to be continuously monitored along the entire microchannel in near real time. This is in contrast to both conventional slab gel imaging where the entire gel can be viewed but only at one point in time after completion of the separation, and capillary electrophoresis systems that permit detection as a function of time but only at a single downstream location read more ...