Hydrophoretic Method for Continuous Blood Cell Separation
Sungyoung Choi and Je-Kyun Park
from: Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis (Edited by: Keith E. Herold and Avraham Rasooly). Caister Academic Press, U.K. (2009)
Precise and rapid isolation of blood cells is of fundamental importance in clinical and biomedical researches. The preparation of white blood cells for downstream analysis is commonly accomplished by selective lysis of red blood cells and differential centrifugation, which are labor-intensive and require a large volume of blood samples. Recent technical advances of microfluidic devices for blood cell separation provide new capabilities for accurate and fast separation of a small number of blood cells. In the microfluidic environment, separation devices take advantage of accurate cell control without turbulent disturbance due to laminar flow at low Reynolds number and high process efficiency even with a small number of cells. This chapter introduces a microfluidic technology to separate blood cells by hydrophoresis, describing details of the separation principle and the individual steps necessary to perform the hydrophoretic separation. We simultaneously present a review of other methods for separation of blood cells, comparing their advantages and disadvantages. Finally, we present some challenges and future trends of microfluidic technologies for blood sample preparation read more ...