Design and Fabrication of Microfluidic Devices for Flow-based Separation of Blood Cells
Lance L. Munn and Abhishek Jain
from: Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis (Edited by: Keith E. Herold and Avraham Rasooly). Caister Academic Press, U.K. (2009)
Enrichment of specific cell populations such as leukocytes and circulating cancer cells from a sample of whole blood is the required first step of many clinical and basic research assays. We are developing microfluidic devices that take advantage of the intrinsic features of blood flow in the microcirculation to separate cells directly from whole blood. These devices consist of simple networks of rectangular microchannels manufactured using soft lithography, a technology that allows rapid development of robust, but relatively complex devices capable of accommodating the flow of even dense solutions of blood cells. Polydimethylsiloxane (PDMS) molding is ideally suited for live cell separations because of the ability to coat the channels with various biologically-relevant molecules and the fact that the sizes of blood cells lie in the range of channel sizes easily produced in PDMS. Here we detail our motivation and methodology for producing separation devices using PDMS molding read more ...