The Leishmania Proteome
Nicolas Fasel, Nathalie Acestor, Amal El Fadili-Kündig, Iveth Gonzalez and Slavica Masina
from: Leishmania: After The Genome (Edited by: Peter J. Myler and Nicolas Fasel). Caister Academic Press, U.K. (2008)
Genome projects for the most medically important protozoan parasites have been initiated in the hope that their sequencing will help to understand the biology of the parasites and to identify new targets for urgently needed drugs, or for the development of vaccines. The completion of the genome sequence of Leishmania major corresponds to the achievement of one important milestone in the quest for such information. Data mining into the sequences of the related genomes of Trypanosoma brucei (T. brucei ), T. cruzi and for other Leishmania species such as L. braziliensis, L. infantum and L. mexicana provides additional tools for the identification, comparative analysis and functional definition of proteins. Taken together, this mass of information opens the door for large-scale proteomic studies to dissect not only protein expression, regulation and function, but also biological processes involving complex protein-protein interactions, post-translational regulation events and post-translational modifications. Considering that gene expression in trypanosomatids is largely regulated at the post-transcriptional level, a systematic analysis of proteins at a high throughput level is an obvious approach. The recent technological developments allowing the rapid identification of proteins by peptide fingerprinting and mass spectrometry have opened the door for such analysis read more ...