Genomotyping of Helicobacter pylori and its Host: Microarray Based Insights on Gene Variation, Expression and Function
Olivier Humbert, Delia M. Pinto-Santini and Nina R. Salama
from: Helicobacter pylori: Molecular Genetics and Cellular Biology (Edited by: Yoshio Yamaoka). Caister Academic Press, U.K. (2008)
Whole genome microarray analysis or genomotyping has been used to query many aspects of Helicobacter pylori biology. Comparative genomic hybridization has yielded new insights into the genome wide extent of genetic diversity both between H. pylori strains from different geographic regions as well as within the bacterial population of single infected hosts. Transcriptional profiling of bacterial RNA has shown that H. pylori has a complex transcriptional response to a variety of environmental stresses encountered in the stomach in spite of having few annotated transcription factors in its genome. Transcriptional profiling of natural (human) and experimental hosts has revealed molecular signatures of disease progression and successful vaccination. Additional microarray based technologies for genetic screens for infectivity, localization of transcription factor binding sites and whole genome mutational analysis are beginning to provide molecular details of the mechanisms by which H. pylori establishes a persistent infection in the human stomach read more ...