Identification of Small Interfering RNA from Next-generation Sequencing Data
Thomas J. Hardcastle
from: Genome Analysis: Current Procedures and Applications (Edited by: Maria S. Poptsova). Caister Academic Press, U.K. (2014)
Small interfering RNAs (siRNAs) play a crucial role in the regulation of transcriptomic and epigenetic factors. Next generation sequencing technologies allow the identification and quantification of siRNAs on a genome-wide scale. Given the proper tools for analysis, biologically meaningful inferences can be made about the biogenesis and patterns of expression of these key components of biological systems. This review discusses the application of sequencing technologies to siRNAs and the currently available tools for the analysis of the data thus generated read more ...