Current Progress in the Sterilization of Spores and Vegetative Cells by Exposure to Gas Plasma: Sterilization, Disinfection and Antimicrobial Activity
from: Gas Plasma Sterilization in Microbiology: Theory, Applications, Pitfalls and New Perspectives (Edited by: Hideharu Shintani and Akikazu Sakudo). Caister Academic Press, U.K. (2016) Pages: 75-90.
In general, spores are more tolerant than vegetative cells against sterilization and disinfection. Thus, spores are generally used as the biological indicator (BI), which should correspond to the microorganism most tolerant to the targeted sterilization process (ISO 11138-1, ISO 14161). Geobacillus stearothermophilus ATCC 7953 was therefore selected as the BI for gas plasma sterilization because its spores are the most tolerant of the tested microorganisms. In this chapter, the relationship between BI and ISO, and their respective importance for validation of sterilization are described in detail read more ...