Current Progress in Advanced Research into Tetrodotoxin Inactivation by Gas Plasmas
Toshihiro Takamatsu, Hidekazu Miyahara, Takeshi Azuma and Akitoshi Okino
from: Gas Plasma Sterilization in Microbiology: Theory, Applications, Pitfalls and New Perspectives (Edited by: Hideharu Shintani and Akikazu Sakudo). Caister Academic Press, U.K. (2016) Pages: 51-58.
This study treated tetrodotoxin (TTX) solution with non-thermal multi-gas plasma and analyzed its decomposition by liquid chromatography coupled with electrospray time-of-flight mass spectrometry. The TTX signal in the mass spectrum was reduced to different levels by plasma irradiations using various gases. Nitrogen plasma exhibited the optimal capability for TTX decomposition, followed by oxygen, argon, and carbon dioxide plasmas. The TTX concentration decreased 100-fold after 10 min treatment with nitrogen plasma. To better understand the TTX degradation process, plasmas of five different gases were generated by a multi-gas plasma jet. The OH radicals and ozone molecules formed at the solution interface were then measured by electron spin resonance and photometry. The largest amount of ozone (64 µM at 15 s) and OH radical (130 µM at 30 s) were generated by oxygen and nitrogen plasma, respectively. We concluded that the generated reactive oxygen species such as OH radicals and ozone contribute to TTX degradation read more ...