Flow Cytometry of Yeasts and Other Fungi
Tim W. Overton
from: Flow Cytometry in Microbiology: Technology and Applications (Edited by: Martin G. Wilkinson). Caister Academic Press, U.K. (2015) Pages: 119-158.
Flow cytometry is used extensively to investigate many aspects of yeast physiology and cell biology. This chapter describes the most common applications of flow cytometry and fluorescence-activated cell sorting (FACS) to yeasts and other fungi, and reviews recent studies to capture current directions in the field. Pure and applied aspects are discussed, with a focus on the areas of fundamental cell biology, medical applications, monitoring during bioprocesses including brewing, and the use of yeast as a molecular biology tool. Novel flow cytometric and microfluidic systems which utilise yeast as a model organism are also described and their relevance to previous research in the area and their possible application for future fundamental and applied research are discussed read more ...