Flow Cytometry and Microbial Community Fingerprinting
Susann Müller and Kathleen Schleinitz
from: Flow Cytometry in Microbiology: Technology and Applications (Edited by: Martin G. Wilkinson). Caister Academic Press, U.K. (2015) Pages: 77-90.
Flow cytometry (FCM) can be used to reveal community patterns by analyzing certain cell parameters that reflect microbial community structures. The method is valuable for separating a microbial community into sub-sets of individuals with shared physiological properties, such as proliferation activity and scatter characteristics. Fingerprinting tools elucidating community composition, i.e. describing the phylogenetic affiliation of the single community members can also complement FCM. These techniques are introduced with a short overview. Their applications for microbial community composition and structure analyses will be compared. The chapter then describes how fingerprinting techniques can be applied to characterize the FCM-separated subsets. This chapter does not provide detailed protocols for applying FCM to microbial communities, as these protocols will vary depending on the community and physiological parameters of interest. Instead, this chapter highlights the general methodology and approach and discusses potential future advances in the field read more ...