Caister Academic Press

The Flavivirus NS3 Protein: Structure and Functions

Dahai Luoa, Siew Pheng Lim and Julien Lescar
from: Molecular Virology and Control of Flaviviruses (Edited by: Pei-Yong Shi). Caister Academic Press, U.K. (2012)


The non-structural protein 3 (NS3) of flaviviruses is the second most conserved amongst the viral proteins. It bears a molecular mass of 69 kDa and is endowed with multiple functions including proteolytic processing, nucleic acid duplexes unwinding, nucleoside triphosphatase (NTPase) and RNA nucleoside 5' triphosphatase (RTPase). Besides these enzymatic activities which are essential for replication of viral genomic RNA, this protein also participates in other aspects of the viral life cycle. Numerous crystal structures of apo- and ligand-bound 3D structures for the protease and helicase domains of NS3, as well as for the full-length NS3 polypeptide (NS3FL) have been determined, providing insight at the atomic level into its various enzymatic activities. In this chapter, we summarize recent progress published regarding the function and structure of this fascinating viral non-structural protein including its recently uncovered dynamic properties read more ...
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