Human Papillomavirus DNA Replication: Insights into the Structure and Regulation of a Eukaryotic DNA Replisome
Claudia M. D'Abramo, Amélie Fradet-Turcotte and Jacques Archambault
from: Small DNA Tumour Viruses (Edited by: Kevin Gaston). Caister Academic Press, U.K. (2012)
Human papillomaviruses (HPV) replicate their double-stranded, circular DNA genome using two virally encoded proteins, E1 and E2, and several components of the host DNA replication machinery. Viral DNA replication is initiated by the recruitment of the E1 helicase to the viral origin of replication, through its interaction with E2, where it assembles into double hexamers and interacts with cellular factors to form an active replication complex. As such, replication of the HPV genome bears many similarities with that of the host DNA at the biochemical level, making these small DNA tumour viruses excellent models for the study of eukaryotic DNA replication. This chapter will provide a detailed view of HPV DNA replication with an emphasis on the different mechanisms utilized by these viruses to maintain and amplify their genome in undifferentiated and differentiated keratinocytes, respectively, and how these processes are regulated by phosphorylation of the E1 protein read more ...