Metabolic Engineering of Corynebacterium glutamicum for Alternative Carbon Source Utilization
Lennart Leβmeier, Christian Matano, Ahmed Zahoor, Steffen N. Lindner and Volker F. Wendisch
from: Corynebacterium glutamicum: From Systems Biology to Biotechnological Applications (Edited by: Andreas Burkovski). Caister Academic Press, U.K. (2015) Pages: 57-70.
Corynebacterium glutamicum is well known for the multi-million-ton-scale production of L-glutamate and L-lysine in biotechnological industry, which is currently based on hexoses derived from starch and molasses. Only recently, metabolic engineering approaches were used to broaden the substrate spectrum of C. glutamicum, allowing access to alternative carbon sources such as glycerol, galactose and pentose sugars present in lignocellulosic hydrolysates. Unlike most microorganisms, C. glutamicum is able to co-utilize mixed carbon sources, making it a preferential host for utilization of complex carbon sources such as wastes from agricultural industry. Several industrial byproducts have been successfully valorized by engineered C. glutamicum strains. Fermentative production of amino acids has been shown based on crude glycerol from biodiesel production, silage juice and rice straw hydrolysates. In this review, we will present a glimpse into the state of the art of metabolic engineering of C. glutamicum toward utilization of alternative carbon sources, such as wastes and unprocessed sources read more ...