Growth and Production Capabilities of Corynebacterium glutamicum: Interrogating a Genome-scale Metabolic Network Model
Elisabeth Zelle, Katharina Nööh and Wolfgang Wiechert
from: Corynebacterium glutamicum: From Systems Biology to Biotechnological Applications (Edited by: Andreas Burkovski). Caister Academic Press, U.K. (2015) Pages: 39-56.
In recent years, the assembly of genome-scale metabolic networks has been established as a powerful tool for obtaining a comprehensive understanding of microbial production hosts and for exploring their industrial potential. Network modeling provides a versatile framework for analyzing, predicting and, eventually, optimizing cellular processes. This book chapter reviews the current state of stoichiometric modeling for Corynebacterium glutamicum and presents a new curated network containing 475 reaction steps and 408 metabolites. This network is interrogated to obtain answers for several fundamental questions on quantitative growth and production capabilities of the organism. Using flux balance analysis the model is validated with experimental data, maximal yields for all 20 amino acids are exemplarily computed and the impact of biomass composition data on model prediction is systematically investigated. As an extension of stoichiometry, the structural prerequisites for metabolic flux analysis are discussed. For the purpose of isotope-based flux analysis, the central metabolic network is extended by carbon transition information required for tracer studies. The genome-scale metabolic network presented in this chapter is supplied via a web server read more ...