Metabolic Network Analysis and Design in Corynebacterium glutamicum
Christoph Wittmann and Elmar Heinzle
from: Corynebacteria: Genomics and Molecular Biology (Edited by: Andreas Burkovski). Caister Academic Press, U.K. (2008)
Methods of metabolic flux analysis and design of metabolic networks for the overproduction of metabolites are reviewed and discussed with respect to their importance for Corynebacterium glutamicum. These methods are relevant for an in-depth understanding of the in vivo function of metabolism and its directed engineering. The methods discussed here are based on solid material balances and well established genomic and biochemical knowledge about metabolic reactions. Monitoring of metabolic fluxes yields very reliable and quantitative genome wide data reflecting the genomic background and environmental influences on cells studied. Particularly flux split ratios between glycolysis and pentose phosphate pathway and activities around the pyruvate node are elucidated that are most relevant for the supply of precursors and NADPH. The paper outlines also methods for design of metabolic pathways optimal for the overproduction of metabolites read more ...