Glycoprotein Trafficking in Virion Morphogenesis
Markus Eickmann, Dorothee Gicklhorn, and Klaus Radsak
from: Cytomegaloviruses: Molecular Biology and Immunology (Edited by: Matthias J. Reddehase). Caister Academic Press, U.K. (2006)
Abstract
Assembled cytomegalovirus nucleocapsids are exported from the nuclear compartment by sequential events including (i) primary envelopment at the inner nuclear membrane and (ii) de-envelopment at the outer nuclear membrane prior to (iii) secondary envelopment of naked cytoplasmic nucleocapsids at cytoplasmic cisternae, and (iv) release of mature enveloped particles by exocytosis. In permissive cells viral envelope glycoproteins are subject to retrograde transport from the site of biosynthesis, namely the rough endoplasmic reticulum, into the inner nuclear membrane presumably by lateral diffusion, as well as to anterograde transport along the cellular exocytosis pathway to the plasma membrane from where they are retrieved to the cytoplasmic compartment of final viral maturation. Recent investigations have unravelled some of the molecular mechanisms involved in cellular trafficking of viral gene products and their interaction with host cell products during virion morphogenesis. Continued efforts along this line will contribute to elucidate pivotal questions of cell biology read more ...
