Burkholderia Proteomics: Methodologies, Challenges, and Applications
Isabel Sá-Correia, Sandra C. dos Santos and Andreia Madeira
from: Burkholderia: From Genomes to Function (Edited by: Tom Coenye and Eshwar Mahenthiralingam). Caister Academic Press, U.K. (2014)
Complete genome sequencing for a large number of microorganisms has paved the way for large-scale proteomic studies to investigate global protein expression levels, regulation and, and to assist in functional analysis. Although microarray technologies can provide invaluable information on genomic expression, it is also known that mRNA levels often do not correlate directly with protein levels. Proteomics is thus necessary to provide complementary quantitative and functional data to genomic information. In this review we will provide a comparative overview of the various proteomic approaches currently available and give a brief tutorial on the methodology and application of two-dimensional electrophoresis-based expression proteomics. Subsequently, the exploitation of proteomic analyses in studies dedicated to different Burkholderia species with a broad range of objectives will be discussed, based on the relevant scientific literature. These include biomedical research and applications (e.g. elucidation of pathogenic mechanisms and interaction with the host, improvement of diagnostic tools, identification of immunoreactive proteins and candidate vaccine targets) and environmental studies, namely on biodegradation of organic pollutants by Burkholderia strains read more ...