Proteomics as a Tool for Metabolic Pathways Inspection in Microbial Cells
Rafał Szewczyk and Konrad Kowalski
from: Microbial Biodegradation: From Omics to Function and Application (Edited by: Jerzy Długoński). Caister Academic Press, U.K. (2016) Pages: 67-84.
Proteomics has become an important part of the characterization of biological systems or single organisms. In this chapter, background relating to proteomics studies including definitions, instrumentation, and basic as well as more complex workflows for protein extraction and identification with different instrumental techniques and bioinformatics tools are described. The application of proteomics in biodegradation is described in terms of selected research studies covering typical approaches: gel-free and gel-based as well as qualitative and quantitative comparative proteomics workflows and metaproteomics read more ...