High-Throughput Approaches to Analyze Waste Biotreatmentin Confined Environments
Ben Stenuit, Laurent Eyers, Spiros N. Agathos and Isabelle George
from: Microbial Biodegradation: Genomics and Molecular Biology (Edited by: Eduardo Díaz). Caister Academic Press, U.K. (2008)
Sustainable development requires the promotion of environmental management and a constant search for new technologies to treat vast quantities of wastes generated by increasing anthropogenic activities. Biotreatment, the processing of wastes using living organisms, is an environmentally friendly, relatively simple and cost-effective alternative to physico-chemical clean-up options. Confined environments, such as bioreactors, have been engineered to overcome the physical, chemical and biological limiting factors of biotreatment processes in highly controlled systems. The great versatility in the design of confined environments allows the treatment of a wide range of wastes under optimized conditions. In this chapter, we provide an overview of current genomic-driven strategies to analyze, monitor and assess biotreatment of different classes of wastes in various confined environments. This overview shows that to perform a correct assessment, it is necessary to consider various microorganisms having a variety of genomes and expressed transcripts and proteins. In addition, a great number of analyses are often required. Using traditional genomic techniques, such assessments are limited and time-consuming. On the other hand, several high-throughput techniques originally developed for medical studies hold great promise. We discuss in this chapter various high-throughput techniques and their possible -or already demonstrated- applications to assess biotreatment in confined environments read more ...