Metagenomics for Viral Discovery in Bats
M. Geldenhuys and Wanda Markotter
from: Bats and Viruses: Current Research and Future Trends (Edited by: Eugenia Corrales-Aguilar and Martin Schwemmle). Caister Academic Press, U.K. (2020) Pages: 191-212.
Metagenomics using high-throughput sequencing technologies can serve as powerful tools to investigate viral discovery in bats and define their virome diversity. Viral metagenomic research has been performed on several bat species in countries from nearly all continents, and the identification of diverse viral sequences has been phenomenal. Viral metagenomics is inherently faced with many limitations and challenges that need to be overcome before and after sequencing results on sequencing platforms are obtained. This chapter reviews the successes of viral discovery from bats using metagenomic approaches, discusses terminology, details the enrichment approaches utilised, describes the challenges encountered and lists several mitigation strategies for poor sequencing results that can be adopted read more ...