Models for Studying Bacterial Pathogenesis
Richard W. Titball and Olivia L. Champion
from: Bacterial Pathogenesis: Molecular and Cellular Mechanisms (Edited by: Camille Locht and Michel Simonet). Caister Academic Press, U.K. (2012)
Research into mechanisms of virulence underpins work to devise improved control measures for infectious diseases. However, the ability of a pathogen to cause disease is implicitly dependent not only on the bacterial species but also on the host. Without the availability of a suitable experimental host, studies on mechanisms of virulence either cannot proceed or cannot be interpreted in an appropriate contextual manner. This chapter considers the advantages and disadvantages of different hosts, including mammals, zebra fish, plants, Caenorhabditis elegans, insects such as Drosophila melanogaster and Galleria mellonella, and cell culture systems for investigating mechanisms of virulence of pathogens which cause disease in humans. The potential for gaining additional information and using fewer animals in experiments involving mammals is reviewed, with a particular emphasis on the potential for new imaging techniques to provide additional information. Much of this information will of course be of relevance to pathogens which are associated with diseases in non-human hosts read more ...