The Metabolic Function of Uncultured Microorganisms Assessed Through Single Cell Genomic Techniques
Dorthe Groth Petersen
from: Applications of Molecular Microbiological Methods (Edited by: Torben L. Skovhus, Sean M. Caffrey and Casey R.J. Hubert). Caister Academic Press, U.K. (2014)
Most microorganisms resist cultivation, which prevent detailed descriptions of their physiological properties. Recent advances in single cell genomics have begun to allow analysis of the genetic content of single cells from hitherto uncultured and inaccessible lineages. This chapter focuses on methods for analyzing single microbial cells such as whole genome amplification, fluorescent activated cell sorting and optical tweezer technologies, and highlights case studies based on these methods. Furthermore, methods for combining isotope labeling and molecular identity within single cells such as MAR-FISH, Nano-SIMS and Raman spectroscopy analyses are also introduced read more ...