Caister Academic Press

Next Generation Sequencing Approach to Understand Pipeline Biocorrosion

Hyung Soo Park, Jaspreet Mand, Thomas R. Jack and Gerrit Voordouw
from: Applications of Molecular Microbiological Methods (Edited by: Torben L. Skovhus, Sean M. Caffrey and Casey R.J. Hubert). Caister Academic Press, U.K. (2014)

Abstract

Use of pyrotag sequencing to determine microbial community compositions associated with corrosion in a pipeline system handling brackish water has shown that different populations are present in different parts of a single pipeline and treatment system. Possible factors influencing the composition of the microbial population include the source of water, flow, time of sampling, mode of growth (planktonic or sessile) and addition of treatment chemicals. Detailed examination of the populations indicated that methanogenic organisms may be responsible for MIC in the upstream water gathering system but downstream of the injection point for sodium bisulfite (an oxygen scavenger) Deltaproteobacteria are more likely the cause of MIC. The mechanism of attack is not necessarily restricted to the widely recognized action of SRB but may also be due to elemental sulfur formed by Desulfocapsa through the disproportionation of bisulfite. These novel insights could not have been obtained through the traditional assay methods currently used in the management of MIC and indicate the promise of molecular methods in future MIC investigations read more ...
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