Caister Academic Press

Biology and Phylogeny

from: Acanthamoeba: Biology and Pathogenesis (2nd edition) (Author: Naveed Ahmed Khan). Caister Academic Press, U.K. (2015) Pages: 1-66.

Abstract

1. Introduction
1.1. Acanthamoeba: a eukaryotic pathogen
1.2. Protists
1.3. Discovery of pathogenic amoebae
2. Discovery of Acanthamoeba spp.
3. Speciation and genotyping
3.1. Use of 18S rRNA gene sequences (18S rDNA) for Acanthamoeba taxonomy
4. Cell biology
4.1. Nucleus
4.2. Cytoplasm
4.2.1. Cytosol
4.2.2. Organelles (organs of the cell)
4.2.3. Inclusion bodies
4.3. Endoplasmic reticulum
4.4. Ribosomal RNA (rRNA)
4.5. Proteins
4.6. Lipids
4.7. Prostaglandins
4.8. Contractile vacuole and osmoregulation
4.9. Lysosome, peroxisome, and digestive vacuoles
4.10. Plasma membrane
4.11. Surface properties
4.12. Carbohydrates: glycogen storage and cell wall synthesis
4.13. Ionic distribution
4.14. Polyamine
4.15. Adenylate cyclase
4.16. Purine and pyrimidine
4.17. Mitochondria
4.18. ER-mitochondria encounter structure (ERMES)
5. Nuclear genome
6. Mitochondrial genome
7. The complete A. castellanii genome (By Professor Brendan Loftus)
7.1. Genome sequencing strategy
7.2. Genome assembly
7.3. Determination of assembly correctness
7.4. Gene finding
7.5. Transcript coverage for the predicted gene set
7.6. Functional annotation assignments
7.7. Genome analysis
7.7.1. Lateral gene transfer (LGT)
7.7.2. Expression of lateral gene transfer in A. castellanii
7.7.3. Lateral gene transfer introns
7.7.4. Unusual metabolism of A. castellanii
7.7.5. Transcription factors
7.7.6. Kinome of A. castellanii
7.7.7. Phosphotyrosine signalling in A. castellanii
7.7.8. Microbial recognition through pattern recognition receptors
7.7.9. Mannose binding protein
7.7.10. Antimicrobial defence
8. Motility
9. Molecular basis of motility
9.1. Intermediate filaments
9.2. Microtubules
9.3. Microfilaments
9.3.1. Actin polymerization to form actin microfilaments
9.3.2. Regulators of actin polymerization
9.3.2.1. Actin-binding proteins
9.3.2.2. Inositol phospholipids-based regulation of actin microfilaments
9.3.2.3. GTP-binding proteins regulate actin microfilaments
9.3.2.4. Other key regulators
10. Acanthamoeba actin
10.1. Properties of Acanthamoeba actin and its filaments
11. Acanthamoeba myosin
11.1. Myosin-I
11.2. Myosin-II
11.3. Activation of myosin-I and myosin-II
11.4. Localization of Acanthamoeba myosin
11.4.1. Myosin-I
11.4.2. Myosin-II
11.5. Myosins and motility
11.6. Polymerization of Acanthamoeba myosin
11.7. ATPase activity of myosin-I and myosin-II
11.7.1. Myosin-I
11.7.2. Myosin-II
Access full text
Related articles ...