PCR Temperature Specifications

from Julie Logan, Kirstin Edwards and Nick Saunders in Real-Time PCR: Current Technology and Applications

Platform	Max. heating/ cooling rate (°C/sec)	Temperature Accuracy (°C)	Temperature Uniformity (°C)
ABI 7900HT Fast	3.0/3.0	+/- 0.25	+/- 0.5
ABI 7300	1.1/1.1	+/- 0.25	+/- 0.5
ABI 7500	0.8/1.6	+/- 0.25	+/- 0.5
ABI 7500 Fast	3.5/3.5	+/- 0.25	+/- 0.5
ABI StepOne	2.2/2.2	+/- 0.25	+/- 0.5
Mastercycler ep realplex⁴	6.0/4.5	+/- 0.2	+/- 0.3
Roche LightCycler	20.0/20.0	+/- 0.4	+/- 0.2
Roche LightCycler 480	4.8 /2.5	+/- 0.2
BioRad MiniOpticon	2.5/2.0	+/- 0.2	+/- 0.4
BioRad MyiQ	3.3/2.0	+/- 0.3	+/- 0.4
BioRad Opticon 2	3.0/2.0	+/- 0.4	+/- 0.4
BioRad Chromo4	3.0/2.0	+/- 0.3	+/- 0.4
BioRad iQ5	3.3/2.0	+/- 0.3	+/- 0.4
Stratagene Mx4000	2.2/2.2	+/- 0.25	+/- 0.25
Stratagene Mx3000P	2.5/2.5	+/- 0.25	+/- 0.25
Stratagene Mx3005P	2.5/2.5	+/- 0.25	+/- 0.25
Corbett Rotor-Gene 6000	15.0/20.0	+/- 0.25	+/- 0.01
Cepheid SmartCycler	10.0/2.5	+/- 0.5	+/- 0.5

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