Recommended reading: Climate Change and Microbial Ecology | Polymerase Chain Reaction | SUMOylation and UbiquitinationPlant genomics is an increasingly important area of science that has expanded in recent years due to the development of advanced technologies and methods. An understanding of plant genomics is a prerequisite for advanced plant breeding and crop improvement. An in-depth knowledge of plant genomics helps researchers to enhance production, confer resistance or tolerance to adverse conditions and improve crops. The recent advances in plant genomics and bioinformatics has had a significant impact on plant science and genetics. New methods and technology have led to a greater understanding of both structural genomics and functional genomics. Plant genomics generates opportunities to create crops with improved traits.
Gene Silencing with Green Fluorescent ProteinThe green fluorescent protein (GFP) of jellyfish (Aequorea victoria ) has significant advantages over other reporter genes, because expression can be detected in living cells without any substrates. Epigenetic phenomena are important to consider in plant biotechnology experiments for elucidate unknown mechanism. In a recent study, soybean immature cotyledons were generated embryogenesis cells and engineered with two different gene constructs (pHV and pHVS) using gene gun method. It was demonstrated that using sGFP(S65T ) as a reporter gene in vector system may be useful for transgenic evaluation and avoid gene silencing in plants for the benefit of plant transformation system.
Antisense Phenotypes in Transgenic RiceOsARF1 is the first full-length member of auxin response factor (ARF) gene family to be cloned from monocot plant. Using quantitative RT-PCR a recent study found that the transcript abundance of OsARF1 was significantly higher in embryonic tissues than in vegetative tissues. To investigate the effect of OsARF1 on the phenotype of rice, a cDNA fragment of OsARF1 was inserted in inverse orientation to the 35S promoter in vector pBin438 to produce an antisense (AS) construction. The AS-OsARF1 construct was transferred into rice (Oryza sativa L. japonica ) calli via Agrobacterium tumefaciens -mediated transformation. Molecular analysis of transgenic plants showed that the functional expression of OsARF1 was inhibited at mRNA level efficiently. The AS-OsARF1 plants showed extremely low growth, poor vigor, short curled leaves and tillered but were sterile. Therefore, the OsARF1 was shown to be essential for growth in vegetative organs and seed development. Further reading: Plant Genomics
- Epigenetics: Current Research and Emerging Trends
- Next-generation Sequencing: Current Technologies and Applications
- Genome Analysis: Current Procedures and Applications
See also: Current molecular-biology books