Peptide Nucleic Acids: Protocols and Applications (First Edition)
Publisher: Horizon Scientific Press
Editors: Peter E. Nielsen The Panum Institute, Copenhagen, Denmark and Michael Egholm Framingham, Massachusetts, USA
Publication date: May 1999
ISBN-10: 1-898486-16-6 (hbk)
ISBN-13: 978-1-898486-16-9 (hbk)
Pages: x + 262
Peptide Nucleic Acid (PNA) is an analogue of DNA in which the backbone is a pseudopeptide rather than a sugar. PNA mimics the behaviour of DNA and binds complementary nucleic acid strands. The neutral backbone of PNA results in stronger binding and greater specificity than normally achieved. In addition, the unique chemical, physical and biological properties of PNA have been exploited to produce powerful biomolecular tools, antisense and antigene agents, molecular probes and biosensors. Important new applications have emerged that could not be performed using oligonucleotides.
This book contains state-of-the-art protocols and applications on all aspects of Peptide Nucleic Acids. Concepts are explained clearly and in practical terms and each chapter contains concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area. An essential text for all molecular biologists, biochemists, geneticists, biotechnologists and nucleic acid chemists wishing to keep up with the latest developments.
Key Features:
• Eminent contributors |
• Up-to-date |
• Wide appeal |
• Background information |
• Step-by-step protocols |
• Detailed applications |
• Useful reference sections |
• Colour figures |
• Illustrated throughout |
Key Topics:
• PNA Synthesis |
• Hybridization-based Techniques |
• PNA Labeling |
• Genome Rare Cutting |
• Duplex DNA Capture |
• Purification of nucleic acids |
• PCR Clamping |
• Antisense Technology |
• Antigene Technology |
Authors Include:
Peter E. NielsenMichael Egholm
Henrik Ørum
LLoyd M. Smith
Ravi Vinayak
Eugen Uhlmann
James M. Coull
Gerhard Breipohl
Bengt Nordén
S. E. Godtfredsen
Joseph Wang
Chapter List
Section 1. Introduction
1. An introduction to PNA. Peter E. Nielsen and Michael Egholm
Section 2. Chemistry
2.1 PNA oligomer synthesis by Boc chemistry. Troels Koch
2.2 Synthesis of PNA oligomers by Fmoc chemistry. Ralph Casale, Ivar S. Jensen, and Michael Egholm
2.3 PNA-DNA chimeras. Eugen Uhlmann, Beate Greiner, and Gerhard Breipohl
2.4 Synthesis of PNA-DNA chimera by MMT chemistry. Ravi Vinyak
2.5 Labeling of PNA. Henrik Ørum, Ralph Casale, and Michael Egholm
Section 3. Hybridization-Based Techniques
3.1 Thermodynamics of PNA-nucleic acid interactions. Anders Holmén and Bengt Nordén
3.2 Application of peptide nucleic acid probes for in situ hybridization. M. Thisted, T. Just, K.-J. Pluzek, J.J. Hyldig-Nielsen, K.V. Nielsen, T. A. Mollerup, H. Stender, O.F. Rasmussen, K. Adelhorst, and S.E. Godtfredsen
3.3 PNA-arrays for nucleic acid detection. S. Matysiak, S. Würtz, N. C. Hauser, H. Gausepohl, and J. D. Hoheisel
3.4 PNA blocker probes enhance specificity in probe assays. Mark J. Fiandaca, Jens J. Hyldig-Nielsen, and James M. Coull
3.5 MALDI-TOF mass spectrometric detection of PNA hybrids for genetic analysis.
Timothy J. Griffin, Wei Tang, and Lloyd M. Smith
3.6 PNA biosensors for nucleic acid detection. Joseph Wang
Section 4. Biomolecular Tools
4.1 PNA directed genome rare cutting. Vadim V. Demidov and Maxim D. Frank-Kamenetskii
4.2 Duplex DNA capture. Vadim V. Demidov, Nikolay O. Bukanov, and Maxim D. Frank-Kamenetskii
4.3 Purification of nucleic acids by hybridization to affinity tagged PNA probes. Henrik Ørum
4.4 PCR clamping. Henrik Ørum
4.5 Plasmid labeling using PNA Xiaowu Liang, Olivier Zelphati, Cuong Nguyen, and Philip Felgner
Section 5. Antisense/Antigene Technology
5.1 Antisense effects in Escherichia coli. Liam Good and Peter E. Nielsen
5.2 Characterization of PNA-dsDNA strand displacement complexes. H. Jakob Larsen and Peter E. Nielsen
5.3 Interactions of peptide nucleic acids with DNA processing enzymes. Kevin D. Raney, Susan E. Hamilton, and David R. Corey
(EAN: 9781898486169)
