Genetic Engineering with PCR
Publisher: Horizon Scientific PressEditors: Robert M. Horton and Robert C. Tait
Publication date: 1998
ISBN-10: 1-898486-05-0 (pbk), 1-898486-12-3 (hbk)
ISBN-13: 978-1-898486-05-3 (pbk), 978-1-898486-12-1 (hbk)
Pages: 219
"An excellent read ..." SGM Quarterly
" ... commendably up-to-date ... we strongly recommend it " Genetical Research
" this is the right book for all those who think they know everything there is to know about PCR - or have such a student in their seminars. " Human Genetics
" will be referred to often and should be readily at hand " Current Microbiology
While there have been myriad books written on site-directed mutagenesis, and portions of several PCR books address the topic, this is the first laboratory manual that focuses on synthetic (as opposed to analytical) uses of PCR. PCR-mediated sequence manipulation gives the genetic engineer such precise control over construction, mutation, and recombination of sequences that it represents a new generation of DNA technology. There are many applications, innovations, and refinements of the use of PCR for synthetic purposes. This entire volume is devoted to cover the topic in breadth and depth and includes a variety of approaches for mutagenesis, recombination, and the construction of synthetic or semi-synthetic genes.
Features include:
• PCR-mediated recombination |
• PCR-mediated mutagenesis |
• Very rapid thermal cycling |
• Control of random error rates |
• DNA splicing by directed ligation |
• Construction of synthetic DNA molecules |
• Antibody engineering with PCR |
• PCR-based scanning mutagenesis |
• In-vitro synthesis of viroids |
• In-vitro selection of functional sequences |
• In-vitro molecular evolution |
• Appendix 1. A primer on PCR |
• Appendix 2. Abbreviations |
• Comprehensive index |
• Fully illustrated throughout |
Chapter List
1. An Introduction to Genetic Engineering with PCR. Robert M. Horton and Robert C. Tait
2. Mutagenesis by Megaprimer PCR. Salen Barik
3. Effects of dNTP and Divalent Metal Ion Concentrations on Random PCR Mutagenesis. Michel Fromant, Sylvain Blanquet, and Pierre Plateau
4. Rapid Cycle Amplification for Construction of Competitive Templates. Russell A. Brown, Marla Lay, and Carl T. Wittwer
5. DNA Splicing by Directed Ligation (SDL). Yuri A. Berlin
6. Multimers of Specific DNA Sequences Generated by PCR. William A. Rudert and Massimo Trucco
7. The Use of PCR-based Scanning Mutagenesis in Molecular Cell Biology. Philip Wulf and Ueli Suter
8. In vitro Synthesis of Viroids. M.A. Rezaian
9. In vitro Selection of Functional Nucleic Acid Sequences. Dan Nieuwlandt
10. Directed and Random Recombination of Antibody Sequences. Ian Lorimer and Ulrich Brinkmann
11. Construction, Assembly and Selection of Combinatorial Antibody Libraries. Richard Powell and Kathryn E. McLane
12. Beyond the Limits of Natural Diversity: PCR Synthesis of Semi-random Peptide and Antibody Phage Display Libraries. Kathryn E. McLane
Appendix 1. A Primer on PCR
Appendix 2. Standard Abbreviations to Represent Ambiguity in Nucleic Acid Sequences
Index
(EAN: 9781898486053, 9781898486121)
