Caister Academic Press

Peptide Nucleic Acids: Protocols and Applications (Second Edition)

Publisher: Horizon Bioscience
Editor: Peter E. Nielsen The Panum Institute, Copenhagen
Publication date: January 2004
ISBN-10: 0-9545232-4-5
ISBN-13: 978-0-9545232-4-4
Pages: viii + 318

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This second edition has been extensively revised, updated, and enlarged to include new material covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of Peptide Nucleic Acids (PNA) and in addition describes the theory and history of PNA. Concepts are explained clearly and in practical terms and each chapter contains concise background information. Written by leading experts in the field, the book is an invaluable contribution to this novel and exciting area. An important reference text and laboratory manual for all molecular biologists.

Key Features:

•   Completely up-to-date
•   Extensively revised
•   Written by leading experts in the field
•   Illustrated throughout
•   Comprehensive reference sections
•   Covers PNA theory and technology
•   Focus on current topics and applications


"This is an extensively revised and updated edition of an important reference text and laboratory manual for molecular biologists ... highly recommended" from E-Streams (May 2005) Vol 8 No 5.

"This book is an excellent guide to PNAs and I would recommend it highly for anyone wanting an up to date and detailed text on this technology." from Australian J. Med. Sci. (May 2005) 26(2): 69-70..

Chapter List:

Chapter 1. An Introduction to PNA
Peter E. Nielsen and Michael Egholm

Chapter 2. PNA Synthesis by Boc Chemistry
Troels Koch

Chapter 3. Synthesis of PNA Oligomers by Fmoc Chemistry
Ralph Casale, Ivar S. Jensen, and Michael Egholm

Chapter 4. Thermodynamics of PNA Interactions
Tommi Ratilainen, Anders Holmén and Bengt Nordén

Chapter 5. Peptide Nucleic Acid Targeting of Double-Stranded DNA
Thomas Bentin, H. Jakob Larsen, and Peter E. Nielsen

Chapter 6. PNA Beacons and Fluoroprobes
Bhaskar Datta, Miaomiao Wang, and Bruce A. Armitage

Chapter 7. Vector Tagging Via PNA
Alex G. Rebuffat, Alessio G. Bernasconi and Brigitte M. Frey

Chapter 8. PCR Clamping
Henrik Ørum

Chapter 9. PNA Directed Genome Rare Cutting: New Developments
Maxim D. Frank-Kamenetskii and Vadim V. Demidov

Chapter 10. PNA Openers for Duplex DNA: Basic facts, Fine Tuning and Emerging Applications
Vadim V. Demidov

Chapter 11. Combined Use of PNA and DNA for Fluorescence In Situ Hybridization (FISH)
Kirsten Vang Nielsen, Sven Müller, Tim Svenstrup Poulsen, Susanne Gabs, Andreas Schønau

Chapter 12. Production of PNA-Arrays for Nucleic Acid Detection
Anette Jacob, Ole Brandt, Simone Würtz, Achim Stephan, Martina Schnölzer and Jörg D. Hoheisel

Chapter 13. PNA Antisense in Eukaryotic Systems
David R. Corey

Chapter 14. Antisense Effects in Escherichia coli
Liam Good, Rikard Dryselius and Peter E. Nielsen

•   Guidelines for Sequence Design of PNA Oligomers
•   Handling and Storage
•   Buffers and Ionic Strength
•   Quantification
•   Melting Experiments
•   Trouble Shooting
•   Nomenclature

How to buy this book

(EAN: 9780954523244)