Caister Academic Press

Protein Expression Technologies: Current Status and Future Trends

Publisher: Horizon Bioscience
Editor: François Baneyx Department of Chemical Engineering University of Washington, Seattle, USA
Publication date: April 01, 2004
ISBN-10: 0-9545232-5-3
ISBN-13: 978-0-9545232-5-1
Pages: x + 532

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Advances in protein expression technologies have mushroomed in recent years. In this book internationally renown authors comprehensively review current and emerging expression technologies. Detailed reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus. An essential reference, this book will enable both new and experienced expression technologists to make educated choices on the technology that is best suited for their needs.

Key Features:

•   Completely up-to-date
•   Internationally renown authors
•   Over 500 pages
•   Fully illustrated throughout
•   Covers established and novel expression systems
•   Bacteria, yeast, insect cell, baculovirus, cell lines


"The book will prove to be extremely useful in any molecular biology lab." from Microbiology Today 31: 149. August 2004.

Chapter List:

Chapter 1
Expression of Extremophilic Proteins
Amy M. Grunden, Donald A. Comfort, Erica L. Malotky, and Robert M. Kelly

Chapter 2
Expression, Folding and Degradation in Escherichia coli
Mirna Mujacic and François Baneyx

Chapter 3
Tools for Metabolic Engineering in Escherichia coli
Christina D. Smolke, Vincent J.J. Martin, and Jay D. Keasling

Chapter 4
Expression Systems in Bacillus
Rob Meima, Jan Maarten van Dijl, Siger Holsappel, and Sierd Bron

Chapter 5
Saccharomyces cerevisiae Protein Expression: from Protein Production to Protein Engineering
Ronald T. Niebauer and Anne Skaja Robinson

Chapter 6
Production of Recombinant Proteins Using the Methylotrophic Yeasts Pichia pastoris and Pichia methanolica
Bruce L. Zamost, Gary J. Lesnicki and Sanjay Jain

Chapter 7
Advances in Protein Expression in Filamentous Fungi
Hendrik J. Meerman, Aaron S. Kelley, and Michael Ward

Chapter 8
Insect Cell Expression Systems
Karthik Viswanathan, Yu-chan Chao, John T. A. Hsu, and Michael J. Betenbaugh

Chapter 9
Heterologous Expression of Genes in Chinese Hamster Ovary Cells
Gyun Min Lee and Sun Ok Hwang

Chapter 10
Heterologous Protein Expression in Methylotrophic Bacteria
Kelly A. FitzGerald and Mary E. Lidstrom

Chapter 11
Secretion or Presentation of Recombinant Proteins and Peptides Mediated by the S-layer of Caulobacter crescentus
John F. Nomellini, Michael C. Toporowski, and John Smit

How to buy this book

(EAN: 9780954523251)