Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 9. Figure 15. The NCode miRNA Universal qRT-PCR workflows. miRNAs are polyadenylated in a carefully optimized, combined reverse transcription step using the SuperScript VILO enzyme formulation and a universal primer. The universal first-strand cDNAs correspond to miRNAs, other non-coding RNAs and mRNAs which can all be analyzed subsequently.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.