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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 7. Figure 3. Outcome of a geNorm analysis on a heterogeneous cancer biopsy sample set. M values (line graph on top) for candidate reference genes start out rather high (1.35 for B2M at the left), then gradually drop towards the more stably expressed genes at the right (TBP and YWHAZ with an M value of approximately 0.5). The analysis of V values (bar chart at the bottom) indicates that 4 reference genes are minimally required for optimal normalization in this heterogeneous sample set (V4/5 is below 0.15, indicating that there is not need to include a 5th gene in the normalization.

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.