Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 7. Figure 2. Typical standard curve obtained from a 6-point 4-fold dilution series for an assay with a good amplification efficiency (E=1.953, efficiency=95.3%) as calculated by qbasePLUS. A high correlation coefficient (r2=0.999) and low error on the estimated efficiency (SE(E)=0.3%) indicate a high quality standard curve from which reliable efficiency estimations can be drawn.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.