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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 7. Figure 2. Typical standard curve obtained from a 6-point 4-fold dilution series for an assay with a good amplification efficiency (E=1.953, efficiency=95.3%) as calculated by qbasePLUS. A high correlation coefficient (r2=0.999) and low error on the estimated efficiency (SE(E)=0.3%) indicate a high quality standard curve from which reliable efficiency estimations can be drawn.

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.