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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 4. Figure 6. Effect of dNTP variation on qPCR. Low, medium, and high dNTP concentrations are shown. In this case, low dNTP concentrations (0.12 mM each dNTP, green) result in earlier Cqs (probably because of more magnesium availability). Medium dNTP concentrations (0.20 mM each dNTP, red) cause slightly delayed Cq values but also slightly higher end fluorescence. High dNTP concentrations (0.28 mM each dNTP, blue) fail to amplify, presumably due to lack of magnesium availability. Total Mg2+ concentration is 2mM final.

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.