PCR Troubleshooting and Optimization Figure
Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Figure from: PCR Troubleshooting and Optimization
Chapter 4. Figure 6. Effect of dNTP variation on qPCR. Low, medium, and high dNTP concentrations are shown. In this case, low dNTP concentrations (0.12 mM each dNTP, green) result in earlier Cqs (probably because of more magnesium availability). Medium dNTP concentrations (0.20 mM each dNTP, red) cause slightly delayed Cq values but also slightly higher end fluorescence. High dNTP concentrations (0.28 mM each dNTP, blue) fail to amplify, presumably due to lack of magnesium availability. Total Mg2+ concentration is 2mM final.
- Metagenomics of the Microbial Nitrogen Cycle
- Pathogenic Neisseria
- Human Pathogenic Fungi
- Applied RNAi
- Molecular Diagnostics
- Phage Therapy
- Bioinformatics and Data Analysis in Microbiology
- The Cell Biology of Cyanobacteria
- Pathogenic Escherichia coli
- Campylobacter Ecology and Evolution