Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 4. Figure 4. Results from UCSC in silico PCR tool showing dual priming to a desired and an undesired site. Although these primers may be good by other measures, they are unacceptable for an assay targeting one product. The predicted amplicons from 4A were inputted into BLAT in 4B. BLAT analysis verified that there are only two products with high homology (95.5%). This would potentially confound the interpretation of sequencing or PCR-based genotyping experiments.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.