Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 4. Figure 2. Amplification curves on a LightCycler 1.5, in channel 1. SYBR Green I provides better signal and earlier Cq values relative to LCGreen Plus. A 92 base pair product from the human ABL gene was amplified in two pairs of duplicate reactions. The LightCycler optical system is optimized for SYBR Green I and fluorescein emission and is not matched to LCGreen Plus. Thus, the lowered signal with LCGreen Plus results from its shorter wavelength emission.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.