PCR Troubleshooting and Optimization Figure
Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Figure from: PCR Troubleshooting and Optimization
Chapter 4. Figure 2. Amplification curves on a LightCycler 1.5, in channel 1. SYBR Green I provides better signal and earlier Cq values relative to LCGreen Plus. A 92 base pair product from the human ABL gene was amplified in two pairs of duplicate reactions. The LightCycler optical system is optimized for SYBR Green I and fluorescein emission and is not matched to LCGreen Plus. Thus, the lowered signal with LCGreen Plus results from its shorter wavelength emission.
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