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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 4. Figure 2. Amplification curves on a LightCycler 1.5, in channel 1. SYBR Green I provides better signal and earlier Cq values relative to LCGreen Plus. A 92 base pair product from the human ABL gene was amplified in two pairs of duplicate reactions. The LightCycler optical system is optimized for SYBR Green I and fluorescein emission and is not matched to LCGreen Plus. Thus, the lowered signal with LCGreen Plus results from its shorter wavelength emission.

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.