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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 2. Figure 6. Comparison of one-step SYBR Green-based qPCR master mix from company A and company B on RimM expression profiles from 48 hour-old bacterial bio-film cells. This represents a typical example of the effect of reagent choice on qPCR. By reagent choice alone, qPCR can behave very poorly (even not at all) to extremely well. Such a dramatic effect on assay performance due solely to reagent choice(s) applies to reverse transcription, amplifying problematic AT- and/or GC-rich sequences and repetitive DNA templates, PCR, RT-PCR and qPCR reactions. The extent of success or failure of any qPCR assay is always, undeniably, a function of reagent choice(s).

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.