Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 2. Figure 6. Comparison of one-step SYBR Green-based qPCR master mix from company A and company B on RimM expression profiles from 48 hour-old bacterial bio-film cells. This represents a typical example of the effect of reagent choice on qPCR. By reagent choice alone, qPCR can behave very poorly (even not at all) to extremely well. Such a dramatic effect on assay performance due solely to reagent choice(s) applies to reverse transcription, amplifying problematic AT- and/or GC-rich sequences and repetitive DNA templates, PCR, RT-PCR and qPCR reactions. The extent of success or failure of any qPCR assay is always, undeniably, a function of reagent choice(s).
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.