Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 1. Figure 7. Continuous monitoring of rapid cycle PCR. A 110 bp beta-globin fragment was amplified from human genomic DNA by cycling between 60 C and 90 C over 15 min in the presence of SYBR Green I. In the center is a 3-dimensional plot of temperature, time and fluorescence. The bottom graph traces temperature versus time over the 15 min of thermal cycling. The far left graph plots fluorescence over temperature and monitors the hybridization state of the PCR product. The sharp decrease in fluorescence on the right of the plot corresponds to the PCR product's melting temperature. The graph on the right is a plot of fluorescence versus time and depicts the increase in fluorescence as the reaction product accumulates with increasing cycle number.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.