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PCR Troubleshooting and Optimization Figure

PCR Troubleshooting and Optimization Edited by: Suzanne Kennedy and Nick Oswald
Published: 2011   ISBN: 978-1-904455-72-1
Price: GB £159 or US 0
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...

Figure from: PCR Troubleshooting and Optimization

Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.

PCR Troubleshooting and Optimization

Chapter 1. Figure 6. The Roche carousel LightCycler (right) and the ABI 7700 (left) were the first two real-time instruments marketed worldwide. Besides the obvious difference in instrument size, the batch size (32 vs. 96), sample format (capillary vs plate), excitation (laser vs LED), detection (CCD vs. photodiode), optical path (fiber optic vs. epifluorescence) and cycle speed (rapid-cycle vs. standard) were as different as the groups that created them. The LightCycler enabled 15-30 min PCR with real-time display of fluorescence during amplification, allowing users to watch amplification curves grow in real-time. The 7700 cycled more samples at once in plate format, but the results could not be observed during amplification and were analyzed only after completion of the run. The LightCycler was introduced with SYBR Green I and dual hybridization probes while the 7700 used hydrolysis probes. The carousel LightCycler became a favorite for rapid development and analysis where turn-around-times were important while the 7700 was adopted by many large labs where processing convenience and batch size were paramount.

Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.