Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 10. Figure 7. The effect of varying template concentration and purity, observed by late amplification and poor reproducibility of melting curves. Replicate DNA extracts from several grapevine varieties were diluted to 10 ng/μl and amplified for microsatellite analysis. In (A), most of the samples amplified early (1) while 3 samples of one variety were delayed (2), suggesting that poor purity led to overestimation of DNA concentration and/or inhibited amplification, leading to higher Cqs. Late and poor reproducibility amplification in real time analysis resulted in lower melting curve reproducibility as shown in the difference plot (B2). Useful websites for HRMA.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.