Published: 2011 ISBN: 978-1-904455-72-1
Price: GB £159 or US $310
An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ...
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 10. Figure 4. Unlabeled probe chemistry. Asymmetric PCR is performed in the presence of an unlabeled probe that is blocked at its 3'-end. Both probe/target and full length duplexes are produced, resulting in two melting regions - one for the SNP region covered by the probe and the other for the amplicon. Shown is a derivative plot after exponential background subtraction.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.