Oligonucleotide Design for In-House Real-Time PCR Applications in Microbiology
from: Real-Time PCR in Microbiology: From Diagnosis to Characterization (Edited by: Ian M. Mackay). Caister Academic Press, U.K. (2007)
Today, real-time PCR has entered nearly every field in the biosciences. Numerous applications of real-time PCR have been described for basic scientific purposes and for diagnosis of hereditary and infectious diseases. Ready-to-use assays for profitable targets are commercially available and can be easily implemented in the laboratory's methods repertoire. However, for special situations or just to reduce expenses, it is often desirable to design a real-time PCR assays by oneself. Although often considered a daunting task, a manageable number of design rules permit the generation of reproducible real-time PCR assays. The basic guidelines for successful real-time PCR assay design are presented here read more ...