High-throughput genetic engineering in Trypanosoma cruzi
Luis Miguel de Pablos
from: Protozoan Parasitism: From Omics to Prevention and Control (Edited by: Luis Miguel de Pablos Torró and Jacob-Lorenzo Morales). Caister Academic Press, U.K. (2018) Pages: 65-76.
There are several reasons supporting the fact that kinetoplastid parasites are good models for solving biological questions: i) these parasites are among the most ancient and divergent eukaryotes, ii) the pathogenicity is dictated by dynamic cell/protein structures specific to this order of parasites and exposed to the host immune system, iii) the life cycle of this parasites can be replicated in vitro or in vivo to a nice extend iv) Their life cycles combine drastic developmental changes in response to environmental cues v) Kinetoplastid parasites can survive as extracellular or intracellular forms in a myriad of host including insects, plants or mammals. Thus, the versatility of scenarios faced by those cells makes them interesting to deep studies using genetic engineering. The emergency of new reverse genetic techniques such us gene editing using nucleases or new approaches for gene flanking homology DNA assembly (fusion PCR or Gibson assembly) for precise integration of different tags have been recently used and applied in the field of trypanosomatids. The aim of this chapter will be to review on advances and approaches designed for successful genetic engeneering on Trypanosoma cruzi read more ...