Serum Proteomics for Studying Disease Pathogenesis and Identification of Disease Biomarkers
Parvez Syed, Sandipan Ray, Kishore Gollapalli and Sanjeeva Srivastava
from: Proteomics: Targeted Technology, Innovations and Applications (Edited by: Manuel Fuentes and Joshua LaBaer). Caister Academic Press, U.K. (2014)
It is important to understand the disease pathogenesis and host immune response for making a correct therapeutic decision. Most of the diseases manifest at the protein level and hence analyzing changes at protein level gives an insight into the pathophysiological condition of the patients. However, identifying such subtle differences in protein expression is not an easy task. Over the decades, various proteomic techniques have been developed to perform serum proteome analysis. In this chapter we discuss the application of gel-, mass spectrometry- and array-based methods for the serum proteome analysis and identification of protein biomarkers. Advancement in proteomic technologies has shown the capability of enhancing the diagnostic potential of the existing diagnostic tools and help in assessing the risk factor. We also discuss the challenges associated with serum proteome profiling using these proteomic approaches read more ...