Plasmid-based Expression Systems for Mammalian Cells
Armin Baiker, Rudolf Haase and Hans Joachim Lipps
from: Plasmids: Current Research and Future Trends (Edited by: Georg Lipps). Caister Academic Press, U.K. (2008)
In contrast to bacteria and yeast no natural plasmids are found in mammalian cells. Therefore many attempts to construct different expression vector systems for mammalian cells have been made in recent years. These vector systems can be categorized in terms of vector administration, mechanisms of vector replication and mechanisms to achieve nuclear persistence of the vectors. In this chapter we describe various transfection methods, construction principles for an optimal vector and the different strategies to achieve nuclear persistence. We then concentrate on the description of various plasmid-like expression systems for mammalian cells based either on viral or chromosomal functional elements. Finally, their application in biotechnology and gene therapy are discussed read more ...