Escherichia coli Cloning and Expression Vectors
from: Plasmids: Current Research and Future Trends (Edited by: Georg Lipps). Caister Academic Press, U.K. (2008)
The introduction of gene technology methods together with the DNA sequencing and PCR methodologies have revoluzionarized basic and applied research in molecular biology involving all kinds of organisms from eubacteria up to humans. Since almost all of the cloning experiments start by insertion of DNA fragments into Escherichia coli plasmid vectors, this chapter describes important cloning vectors from the very beginning up to the present days. Furthermore, it deals in detail with expression vectors and the promoter elements needed for high-level production of recombinant proteins. The last part describes special vectors allowing for the analysis of promoters and terminators, addition of localization and epitope tags read more ...