Next-Generation Sequencing Sheds New Light on Small RNAs in Plant Reproductive Development
from: Next-generation Sequencing and Bioinformatics for Plant Science (Edited by: Vijai Bhadauria). Caister Academic Press, U.K. (2017) Pages: 143-170.
Reproductive development is a key step of the plant life cycles and indicates the start of a new life cycle. The reproductive organs including flower, fruit and seed, have diverse and complex structures, which is a syndrome in the evolution of angiosperms. The development of plant reproductive organs depends on the correct spatial and temporal expression of numerous genes acting in concert to form regulatory networks. Small non-coding RNAs (sRNAs) play a key role in the reproductive development through different modes of sequence-specific interaction with their targets. The sRNAs guide transcriptional and post-transcriptional regulation to intensively integrate into the complex process. Next generation sequencing techniques (NGS) has greatly extended scientist's capabilities to identified and quantify sRNAs by supplying a massive of sequences. In turn this has led to a greater understanding of the many complex roles and interactions that sRNAs are involved with during reproductive development. In this review, we provide an overview of the biogenesis and classification of plant sRNAs, and summarize the recent progress in the understanding of plant sRNA in the flower, and fruit/seed development. Also, we discuss NGS approaches to profile global sRNA expression, as well as the application of sRNA-seq/degradome-seq approaches to identify novel sRNAs and verify their targets related to the above development processes read more ...