Manipulation of Nitrogen Metabolism and Alternative Nitrogen Sources for Corynebacterium glutamicum
Nadine Rehm, Julia Bürger and Andreas Burkovski
from: Corynebacterium glutamicum: From Systems Biology to Biotechnological Applications (Edited by: Andreas Burkovski). Caister Academic Press, U.K. (2015) Pages: 71-82.
Nitrogen is an essential component of nearly all macromolecules in a bacterial cell, e.g. proteins, nucleic acids and cell wall components and their corresponding building blocks. To satisfy the nitrogen demand and to ensure a sufficient nitrogen supply even in situations of nitrogen limitation, microorganisms have evolved sophisticated uptake and assimilation mechanisms for different nitrogen sources. This review focuses on nitrogen metabolism and its control in the biotechnology workhorse Corynebacterium glutamicum, which is used for the industrial production of almost four million tons of L-amino acids each year. Ammonium assimilation and connected control mechanisms on activity and transcription level are summarized and the influence of mutations on amino acid pools and production of L-glutamate, L-glutamine and L-lysine is discussed read more ...