Caister Academic Press

Developing Interpretation of Intracellular Metabolism of Corynebacterium glutamicum by Using Flux Analysis Technology

Tomokazu Shirai and Hiroshi Shimizu
from: Corynebacterium glutamicum: From Systems Biology to Biotechnological Applications (Edited by: Andreas Burkovski). Caister Academic Press, U.K. (2015) Pages: 25-38.


Corynebacterium glutamicum is often used as a model organism because of its ability to produce various useful substances. For achievement of high productivity, metabolic flux analysis (MFA) is a powerful technique, which is used to determine the state of intracellular metabolism in microorganisms. In order to develop the precise MFA, it is necessary to obtain 13C labeling information of intracellular metabolites, in addition to the data of extracellular measurements such as substrate consumption rates, cell growth rates, and production rates. Metabolic flux can be estimated accurately through a mathematical approach by using computer calculations to analyze 13C labeling information of intracellular metabolites by performing nuclear magnetic resonance and/or gas chromatography-mass spectrometry. The accurate determination of intracellular metabolisms by using MFA permits a deeper understanding of C. glutamicum physiology and can produce information for improving the production of useful compounds by using this bacterium read more ...
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